I was curious if anyone was using the CellProfiler Worm Pipeline that identifies and measures the worm’s dimensions? I saw online that it uses OilRed staining and that’s how the software identifies the worms.
Is the use of fluorescence required by CellProfiler to identify the worm? I was wanting to try it out on Xenopus embryos (around Stage 24-26) to help me quantify phenotype. I guess I could possibly micro-inject a fluorescent dextrans if the software requires it and could recognize it…
Has anyone used CellProfiler to image and measure Xenopus embryos?