I am trying to perform image analysis with tile images (10x, 9 fields) from immunofluorescence experiments.
Basically we using the dapi signal to detect the nuclei and we quantify the red and green signals corresponding to two different antibodies. We are using this with single field images 40x with no problem.
However I have problems first with metadata, it seems that several images are detected as you can see here.
Thanks in advance for any tips