Right now I have a pipeline that looks at the average of several images (frames from an imaging session) to produce ROI’s around the cells as objects. I use the average of all images because there is ever so slight movement that I want to include.
Anyway, I want to apply this ROI to the series of ~400 frames that are individually saved as tifs in a folder. How do I use the MeasureImageIntensity tool to produce the average intensity of the objects (ROIS) across all 400 images without having to manually input all 400 images in the MeasureImageIntensity step? Right now this step spits out one AverageImageIntensity, and I want it to spit out 400 - one for each image in a table
Thank you in advance for your help!