Ah right, so from that text file I can see that your dimensions should be 0.325 x 0.325 x 1. I got that from the line “Voxel size: 0.3250x0.3250x1 micron^3”.
I tried your pipeline but just changing to those dimensions and still got the error so then I re-saved your Tiff in FIJI with the correct dimensions as below and then loaded that into CellProfiler and it ran through fine. So I guess even though you write in the dimensions in the NamesAndTypes module CellProfiler is also checking the metadata too.
I also noticed that in the Metadata module you aren’t extracting the multiple slices which I think is also needed for CellProfiler to run this properly. So if you click “Add another extraction method” then “Extract from Image File Headers” and click “Update Metadata” button and then “Update” button on the table, you should then see the table populate with all the slices and channels (so for this file you should have 64 rows).
Hope this information helps! Let us know how you get on.