First post here!
A quick question -
Planning to use IdentifyObjectsManually in CellProfiler to create nuclear and cell segmentation masks.
Can anyone confirm whether outlines are meant to be drawn outside or inside objects? i.e. should I be drawing my outline on pixels I deem to be contained at the outermost edge of my object but still part of my object or the pixels which sit directly outside my object?
Hope that makes sense!