It’s actually very simple (I assuming you are aware how to create a pipeline in CP):
- Load your images
- Module Identify Primary objects:
a) Select the input image -> img1 (in your case)
- Mesure Object Intensity:
a) Select an image to measure -> img1;
b) also click “Add another image” under the drop down menu with image selection and select img2, click again for img3 and img4;
c) Select object to measure -> your primary object;
- Measure Area Shape: same as a-b for intensity
- Save results
In the output file you’ll have columns for intensities and areashapes with “_img1”, …, “_img4” endings