CellProfiler for multiplex immunofluoroscence


I have to analyse the images from Leica. My images would have 3 fluorescent tags along with DAPI (in triplicates). I need the results as a spreadsheet with the number of cells per slide and fluorescent intensity of each tag.
I am new to CellProfiler. I have version 3.1.8 installed in my Windows system.
I need help in building a pipeline, that would read the microscope images and give out a spreadsheet with the information required.

Thanks in advance.

There are plenty oftutorials on the CellProfiler web site. There’s also another one here.

1 Like