Cellprofiler for flouresent cell counting

Greetings from Vietnam. I am very new to Cellprofiler. Anyone can help me to improve the pipeline to count and measure the positive green cells.

Thank you so much.

Pipeline1.cpproj (1.0 MB)

@namhuynhnc,

Great work thus far on your pipeline! You seem to be doing a pretty good job of detecting GFP+ cells. Do you have a specific aspect of the image analysis process that you’d like to improve? Is there a specific way that the pipeline is failing? Perhaps you could annotate an image to show what isn’t working well.

Thanks and good luck!
Pearl

Thank you so much. I feel everything is fine now with the results.