Cellprofiler for flouresent cell counting

Greetings from Vietnam. I am very new to Cellprofiler. Anyone can help me to improve the pipeline to count and measure the positive green cells.

Thank you so much.

Pipeline1.cpproj (1.0 MB)


Great work thus far on your pipeline! You seem to be doing a pretty good job of detecting GFP+ cells. Do you have a specific aspect of the image analysis process that you’d like to improve? Is there a specific way that the pipeline is failing? Perhaps you could annotate an image to show what isn’t working well.

Thanks and good luck!

Thank you so much. I feel everything is fine now with the results.