Hi Image.sc forums,
I am using cell profiler to process image masks for nuclei and cytoplasm (generated in Image J) to measure protein concentration.
A certain number of cells are showing two nuclei for a single cell, which is in error. This is due to tissue artifacts and is produced by the process of generating masks in Image J.
Is there any way to tell CellProfiler to discard the object if the a secondary objects contains two or more primary objects?