Hey everyone, I’m very new to CellProfiler and imageJ. However I just started a new project that requires me to use either for my confocal microscope image analysis. After going through the litreature, I have concluded that it is better to use for high throughput image analysis. What I intend to analyse is nuclear morphology of human cells. But i woul like the software to recognize cells, and recognize nuclei inside the cell, count them, and report how many nuclei are in every cell. Also I would like some additional info about these nuclei and cells, such as their size, the ratio of their sizes between each other, the circularity of the nuclei, and the surface area of both cells and the nuclei inside it.
I would appreciate if someone would take a bit of time to help out!