Hi, I am an undergraduate student in Univ of Sheffield, in UK.
I am doing a dissertation on evaluating cell tracking tools, and CellProfiler is one of my test candidates.
I have been trying to use the tool to track cells in images(jpeg). I tried creating the pipeline myself but found some difficulties.
I need to identify two different types of cells; ones in green and ones in red.
I have attached my .cp file and one of the image file.
Could I please get some help in building appropriate pipeline to track the cells in a given area(using crop).
p.s. I have been trying to understand the tracking methods within the tools, however I just did not really understand them. I am not doing a biological science or mathematics but im studying enterprise computing; I have a bit of programming language understanding but not at the lowest level.
It would be very very very helpful if I could get some basic description of methods and what they are advantageous at. Thanks.
DefaultOUT.mat (47.9 KB)