I am about done imaging. I have gotten Cell Profiler Analyst to work before very nicely.
However, this time I have to create the objects manually as opposed to creating them within the PrimaryObjects and Secondary Objects Modules.
My question is, will I still be able to classify objects with the Cellprofiler Classifier if I create the objects from within the IdentifyObjectsManually Module?
Any feedback appreciated!
Thanks very much!