CellProfiler 2.2.0 crashes on startup Windows10

Dear CellProfiler Community,

I wondered if somebody might point me in the right direction. I’m trying to install CP2.2.0 on a Windows 10 machine. I first installed JDE 8u211 and then CP.

However - when I launch CP, I see the black dialogue for a maybe a second and it instantly quits?

Best wishes,

Sorry to hear it! Hm, there are a lot of threads about Windows 10 and various versions of CellProfiler… have you tried any troubleshooting based on those yet?

Often it’s a java issue of some sort…

Dear Anne - Thank you so much for your swift reply. I had a quick search through previous posts here, but perhaps with insufficient diligence… I did note the one you linked, but the solution ‘… System Variable JAVA_HOME set to a 32-bit java installation…’ was slightly beyond my technical grasp. Probably not with some input from google however…

Anyway, thanks for letting me know the issue is likely with java - I will investigate this and try to fix / will post back with an update.

Finally - a huge thank you for everything you and the CellProfiler team do to provide with accessible tools for bio image analysis. (I’m one of Paul’s minions btw! : ))

Best wishes - John

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In case useful for future users, I uninstalled CellProfiler and JDE from the machine. I then installed the Java SE Runtime Environment 8u211 and set the system environmental variables ‘JRE_HOME’ to the x64 java path and reinstalled CellProfiler. Everything now runs great.

Many thanks for pointing me in the right direction Anne.


Wonderful! Glad that did the trick, thanks for reporting back, it’s really helpful when people do so.