Cellprofile for couting astrocytes

Hello,

I could use some help, I am trying to find an automatic method to count the total number of astrocytes in hippocampus, the problem is that they have an elongated shape and the density is very high. At the moment I am using IF pictures.

If anyone has performed something similar I. would appreciate some insight

Here is an example of a cropped picture. Astrocytes hip.tif (4.1 MB)

Thank you