I’m still learning how to measure using CellProfiler. I wanted to quantify the number of muscle cells and measure/outline their shapes on different topographies. I also want to save a file with the outline on those cells for my research.
I also wanted to measure roundness of cells versus the ones with cytoplasmic extensions.
I am trying to run a modified version of shaping pipeline but it doesn’t seem to work with my images. DL_classify_shapes.cppipe (9.1 KB)
I am uploading an example image of my data, could you please assist how to move forward?
I noticed my .tif images are not visible on the forum so I’m uploading the jpeg counterparts.