Hi, I am currently a student in a lab where we use fluorescence microscopy. For our current data analysis process, we measure the fluorescence of cells by using HCImage and manually selecting cells in the time lapse video (scrolling through the video and selecting cells that are lighting up). There are generally 50-100 cells per sample in each time lapse video so this process takes 5+ minutes and is tedious. Is there any way to automatically select these cells/ROI using a specific program? I am hoping to use a program that is suitable or specific for time lapse data and can measure the fluctuating fluorescence for each automatically selected ROI.
I do not have much machine learning or cell segmentation experience so specific recommendations and steps are very appreciated. I do not have a time lapse video example to share, but I have shared two images; one at time 0 seconds and another at time 6 seconds.
*Images removed at request of author.