My goal for downloading this software was to quantify the proportions of cells in a culture. More specifically, I stained them with green and red, and I want to know the ratio of green to red cells in the image. I saw online that there was a Tissue Neighbors download, and I did this but the output is not exactly what I needed. Is there any way to use an image that looks just like the sample image for the Tissue Neighbor, send it into the program, and have it count how many cells are red and how many are green? Also is this tool able to handle movies?
Thanks in advance.