Perhaps you know if it’s a camera setting, camera software setting, or something with Cell Profiler.
I took some images, trying to increase the red with the camera software before taking the picture.
I am trying to separate blue from red with a camera, and not with separate filters (using a DAPI/TRITC filter).
The image looks ok when opened with Preview or GIMP, but when the input folder is selected and I open the image using the lower left menu in Cell Profiler, it looks like it probably would if I had never made the change with the camera software.
I’ve uploaded one of the offending pictures, and the cell profiler screen shot.
Any thoughts on what caused this and/or how to fix it?