Cell Profiler Analyst fetching gray images

Usage & Issues
#1

I am fairly new to Cell Profiler as well as Cell Profiler Analyst. I have used Cell Profiler (LoadImage, ColorToGray, IdentifyPrimaryObjects, ExportToExcel, ExportToSpreadSheet) to create DefaultDB.properties, DefaultOUT.mat, DefaultDB.db, DefaulOUT_Image.csv, and DefaulOUT_Nuclei.csv. When I used .properties file in the CP Analyst’s Classifier, I was fetching gray unclassified images.

Is it possible to fetch sample unclassified images from original multi-channel image, and use xy coordinates for objects from gray image?

Thank you in advance,
Olexandra
test.zip (471 KB)

#2

Hi Olexandra,

When you try to fetch images (and fail), could you copy/paste the text that appears in the black area under the buttons in the CPA main interface? I’d like to see what sort of error message it’s reporting…

Regards,
-Mark