Cell Painting with other Cell Lines

Hi,

has anyone used the Cell Painting protocol successfully with other cell lines than U2OS and A549?
Esp. interesting would be lines that give different results for a given set of compounds (e.g. different compounds are active).

Many thanks in advance.

Kind regards,
Axel

Hello! The Bray et al paper about Cell Painting lists many more cell lines/types that have been successfully Cell Painted. Out of the dozens I’ve heard, the stain concentrations generally just work and no tweaking is needed.

There’s a public Cell Painting wiki with other tricks and tips here: