I am a student of the master’s course in Japan.
I cannot figure out how to generate rules file as text format by CPA for applying it to FlagImage module of CP.
I am trying to do Cell Paint assay referring to [Bray et al. Nat. Proc. 2016] and [Bray et al. J. of Biomol. Screening, 2012].
Now, I have a question about quality control, especially FlagImage module.
I cannot figure out how to generate rules file as text format by CPA for applying FlagImage module of CP.
I would like to eliminate saturated or blurry images from my images.
I can generate property files with the quality control pipeline provided in [Bray et al. Nat. Proc. 2016] .
Also, I can classify images with Classifier of CPA, and evaluate blurry threshold from histograms visually.
Would anyone give me some advice?