Cell Identification

cellprofiler

#1

I having a difficulty to identified the cell in my image. I also planning to differentiate the cell with less intensity color (blue circle) and high intensity color (red circle). The pipeline I developed fail to detect the cells. Any suggestions or ideas?

In_Vivo_MN.cpproj (408.2 KB)


#2

Hi,
I think this will work a bit better for you if you try using UnmixColors instead of ColorToGray- see attached. Good luck!In_Vivo_MN_UnmixColors.cppipe (8.2 KB)