I have many confocal images in lsm format. The images have cells which have been stained with three dyes.
Two dyes are for two different proteins which translocate from cytoplasm to nucleus and thats what the goal is to track their translocation pattern. However, 3rd dye was used to mark the nuclear boundary NOT by staining nucleus BUT by mitochondrial dye which simply outlines the nucleus. So, all three dyes are cytoplasmic, two of them will show the translocation pattern.
How can we work with these images as boundary detection is first step to move-on?
Plz guide me.