Hi, I’m pretty new to IHC and I’m currently trying to analyze my data. I have brain tissue slides that have been stained with DAB and counterstained with DAPI or hematoxylin. I counterstained my slides with DAPI first and if DAPI did not work, I used hematoxylin. I’m trying to quantify the percentage of cells that are DAB-positive.
I tried using the positive cell detection in QuPath and it seemed to work fine with DAB/Hematoxylin, but did not work with DAB/DAPI.
I have QuPath and ImageJ installed, so if you have any suggestions/links on how to quantify DAB-positive cells in either software, that would be great.