Cell counting with identifyprimaryobject module help

I am very new to cell profiler and am having trouble using the identify objects module to actually count the cells I am looking at. Every time I try different settings in this, it doesn’t identify the cells which seem pretty clear in my images. Do i need to do image processing first or are there any recommendations for the pipeline attempts for the attached pictures?


You usually can’t attach images to your very first post- can you try uploading again?

500um 29hr016.tif (9.3 MB)

Just did now, can you see what you think? Thanks

I think you must threshold your image (Image -> Adjust -> Threshold). You may find the better way to highlight only your cells before runinning analyze
Your is that your background is too similar to the cells.