I’m trying to use cell profiler to get an accurate cell count via DAPI staining as a first step in my pipeline. Is this possible? The other method of intensity and shape are difficult as I’m trying to count primary pancreatic islet cells and they are very close together and thus causing error. I figured since I can see the DAPI clearly it would be the best way. Thanks!
Certainly – counting cells with DAPI is a reasonable and typical approach.