Cell counting Fiji,ImagJ,Knime people

Hello Experts,
Im checking translocated images some of them were translocated others not, so how could I do count for translocated and non-translocated cells through Image/Fiji step by step. I have a general idea about Fiji different plugins, but I want to apply these plugins to my images for one photo then I might be able to apply these steps to other images.
Kind appreciation
107

Hi @Biododo

you might have a better ans easier time with CellProfiler

https://cellprofiler.org/tutorials/#cellprofiler-written-tutorials

There is a tutorial there for translocation assays that does just want you want.

Hope this helps

Oli

1 Like

Dear Oburri,
Thanks for your reply but I couldn’t install the program, I received once the black screen for one of the versions and another message for another version. You can see them in the attached photo…

Kind regards
cell%20profiler Doaa