Hi everyone, we are trying to process drosophila images, and we could use some help to get the information we need.
We are trying to count the number of cells and the intensity of each cell along its boundaries.
Input Image (662.6 KB)
The output we are expecting is something like this + Intensity along the sides of each cell (Raw Intensity)
Expected output (1.9 MB)
This is the first time we are trying to do such an analysis, so we are unaware of the tools used for this. We tried cell counter in ImageJ, but the results were not optimal. We are happy to use any new software or analysis tool for this, so if anybody can guide us through or provide a solution, we will be very thankful.
If any more information if required from our end, we will be more than happy to provide it.