I need some help for my experiment.
I have to analyse neuronal markers (GAD2 and GAD67). I did a containing with this 2 markers (cytoplasmic markers) and now I would like to know how much cell expressed GAD2, how much expressed GAD67 and how much expressed both. I attached a picture of the 2 staining. (on this picture there are not many but in the other yes that is why I can’t count it manually)
Could you help me in this way ?