I’m new to CellProfiler and I’m having some diffuclities.
I need to count cells (macrophages) after a migration assay using a Boyden Chamber.
I tried to build a pipeline but the problem is that the identifyprimary doesn’t identify cells but background, and when I invert the pic with ImageMath/Invert result is the same. I don’t understand why. How can I identify all of the cells in the picture?
I attached a relevant picture as example. Cells were stained with Eosin and Methylene Blue, but picture is in gray scale as we only have a black-and-white camera. Small circles we can observe on pic are migration-membrane-pores and should not be interpreted as cells.
I would really appreciated any help.