I’m trying to use cellprofiler to take the original blood cell image -
, crop and rotate to only look at the area in the black rectangle of the top image, and then count the cells. The first image should return 17 cells which is the manual count, but as the cell images arent hugely distinguishable it’s having trouble identifying them and also seems to be incorrectly confusing parts of the grid as cells (as they are a similar colour).
Can anyone advise how I can alter the pipeline so that it only looks for spherical objects and ignores the gridlines? Any help would be hugely appreciated, attached is my pipeline attempt attempt.cp (8.55 KB)