Cell Count - Membrane Stain Alone

Dear Expert,

I am a new user, could you please help me with a pipeline to count these (see as attached) membrane stained cells. There are about 8 cells in the attached image.

Thank you,
Audrey


Hi Audrey,
Assuming that the cells of interest are the dark, semi-circular ones, I’ve put together a pipeline which seems to detect them.
Hope this helps!
-Mark
2014_11_03.cppipe (9.18 KB)

Hi Mark,
Great thanks, the basic pipeline was exactly what I needed.
An additional question came out while I further developed the pipeline, as the size of the image attached previously was only about 1% of the regular size of my images, is there a module that I can use to separate a giant image (2-4 GB each) by gridding it as tiles, and then analyze each tile independently or separately? I have tried the pipeline of the “Yeast patch identification” from the “Specialized Pipelines” but it did not work simply because it does not allow individual tile analysis.
Thank you ahead!
Audrey