I’m recently trying to use CellProfiler to count cells on histological images (hematoxilin&eosine stain)of bone graft on rats with and without MSC.
It has been very dificult to create a pipeline to do the count. I already tried to analize this images on ImageJ using the ITCN plugin, but at first I coundn’t do it, because the program didn’t recognize between osteblast, graft, bone, blood cells. So I used Photoshop to isolate the nuclei of osteoblast firts and then make the count, with very successfull results (compared with manual count). But this photshop process is very hard and sensitive to human error, so the calibration took a long time.
My question is if can CellProfiler make the count and isolate the cells of my interest (osteoblast, osteoclast and fibroblast) on the original images without the photoshop process?, or if it doesn’t, how would be the pipeline to do the count on the images processed with photoshop?.
I attached an example of the original image and the same image processed on photoshop. The manual count of this image is 556.
I’ll be very gratefull for your help.