I’m looking for advice on how to automatise cell area measurements on my data sets which are phase contrast images from a 12 hr timelapse.
So far this has been done by hand but it is very time consuming and not exactly precise and I am looking for ways to try and make it automatic and more precise/unbiased. Before switching all data acquisition to fluorescent images and repeating a number of experiments I want to try and find a way to work with these datasets.
I have so far tried using ImageJ to play around with detecting cell edges but it hasn’t worked well. Is there a way to maybe create a pipeline in Cell Profiler or other software? What kind of image processing functions should I be looking at?
p1_t0.tif (2.8 MB)