Cell Analysis: identify primary objects failed

Hello everybody,

I am new to the forum and also very new to image analysis, but I was wondering if someone could offer me some help. I have images of mouse retinal pigment epithelium (RPE) stained with phalloidin to visualise the actin filament and therefore to visualise the hexagonal shaped RPE cells (see picture attached). I am interested in analysing the following parameter:

  • counting the neighbour cells
  • ObjectSizeShape

I already tried to modify existing pipelines but it fails when it come to identifying these hexagonal shape cells.
Any help or advice I can get on this process would be appreciated, many thousand thanks!

Hi @awolf0,

Hope you had a chance to try some example pipelines from the cellprofiler website. You can explore tissue neighbors example from there.
If still not able to solve, if possible share the pipeline.