I am trying to create a pipeline to analyze heart sections stained with wheat germ agglutinin to measure cardiomyocyte cell diameter and area. However I am having difficulties with the pipeline because some of the cells are broken so the program does not count them as cells. Can you help me with this issue please. I’ve attached the image I am trying to analyze and the pipeline I’ve made so far.
WGA pipe modified.cpproj (441.2 KB)