We’re extremely happy with the CellProfiler program and are using it for doing high throughput image analysis in our lab.
But just a small question: The CellProfiler user manual says there are two ways of doing automated thresholding, Otsu and MoG. We are using the Otsu Global approach and this works great for us. Somebody told me however that the Canny algorithm works better, is this true? And if so, why is it not implemented in the CellProfiler?
Thanks a lot for your help,