I mean what are common artifacts that should be considered before doing image analysis in cell biology?
I think that really depends on the type of imaging you’re doing. There’s no encompassing answer for this.
For example, if you’re doing a bunch of multicolor sequential imaging, you might have to do some registration to make sure everything is aligned appropriately. Another one would be correcting for field distortion or warping if your system is prone to that. The list is endless, any one of us could go on for hours about that.