Thank you for testing with some .dng files!
So, if I am understanding correctly:
If I open my .dng images with Bio-Formats I am opening and processing the original images.
The images that I am working with are displayed as 4032x3024, 8-bit, 35MB (not the 302x44032,16-bit, 70MB as a .tif file would show up as). This is because they undergo a Lossless JPEG compression.
I know that JPEG compression that is lossy creates artifacts and discards some of the pixel data. With my images, I have less bit depth than I would have if analyzing a .tif image, but since the JPEG compression that is shown in the .dng file is lossless, is it correct to assume that I am not losing any more pixel data/image information and should be able to generate data that is being losslessly compressed in the same way each time?
Thank you again for your help! I have a very rudimentary understanding of the level of image quality needed for reliable analysis, so I’m trying to learn exactly what is happening to the images from capture to analysis.