Dear CellProfiler community,
I am currently investigation the possibilities to automatically count a series of live cells images grown of a special coating. Although cell counting may appear as a simple problem to overcome using ImageJ or CellProfiler, I am running into specific problems. As I mentioned before, the cells were grown on a special coating. This makes sure the cells attach and grow as a mono-layer. However, this also results in a “grey speckled” background. Since the cells I am trying to count display the same aspect, I have a hard time differentiating cells from background.
These examples show the difference in morphology and density of the images. I’m trying the avoid having the count these cells manually, as you can probably understand.
Before I really dive into the depths of CellProfiler I ask you:
- Is there a way to set CellProfiler to discard the speckled background?
- Is there enough contrast between background and cells to accurately distinguish them?
Your feedback is very much appreciated!