Calibration for Batch Measurements

Hi there,

I’m working on leaf shape variation and am using imageJ to measure leaf area, perimeter, circularity etc. I’ve been uploading scanned images and batch-measuring the parameters. However, I would like to use a scanned image of a known object e.g. a post-it, with known dimensions as a calibration for the image size but am unsure of how to do this. Could anyone point me in the right direction?

Thank you so much!

Cynthia

Hello,
What type of backing are you using for the scan? If it is a particular size backing. then place a ruler on the backing and scan that. That will give you a scale based on the backing which can be extrapolated to the other images.
Bob