Calibration bar of flurorescent intensity-how to make global to apply all images and modify values on the bar

[kavator (keionn)]

Hi folks
quite new to fiji and was hoping to get some advice regarding the calibration bar in fiji.
is there a way to set scale of calibration bar to apply to all images to be analyzed? (something similar to the likes of a scale bar global option)
Also; how do i modify the values of the calibration bar to represent specific colors? (actually i want to invert the colors of the calibration bar)
hope to get some suggestions!
*will put up a sample image later

[oburri (Olivier Burri)]

Hi @kavator

Is this related to your needs?

Split colored height map of moon in greyscale images

Hi, I’m new to imageJ, I used it in the past to convert some binary data to images. Now i want to do something completly different. I found this nice picture of the moon on the NASA website. [image] and i want to do some art from it. For this i like to split the heightmap in let’s say four images, each represening a certain range (i.e. -9150 to -2000, -2000…0,0…2500…). The LUT is embedded into the image and i want to use this LUT somehow to get several greyscale heigth maps for further pro…

@kavator

*will put up a sample image later

That will certainly help