I do appreciate for inventing and improving the fantastic software.
I’m trying to quantify effects of knockdown of target protein on structure of intermediate(keratin) filaments in cell colonies(in specific, epithelial tubules).
I found two phenotype changes which I want to quantify: decrease of intensity, and defect of filaments orientation.
Now I’m facing trouble with latter one, calculating(converting) orientations of filaments to relative orientations to the ones of colony.
I tried to use CalculateMath module to calculate relative orientations, but it doesn’t work like I wish; it only calculates relative orientation of first one of all filaments[objects].
How can I calculate orientation of all the filaments?
If you also have idea to redefine range of angle to between -90 degrees to 90 degrees, please tell me.