I was wondering if there was a way to calculate multiple nuclei in myotubes or multiple nuclei in any object for that matter. I saw a posting of it before but that was for Cellprofiler 1.0 and there was no good solution. Ideally I would like to be able to tell the number of nuclei per myotube but I don’t see a good way to do that. At least I would like to calculate the number of nuclei within myotubes as opposed to total nuclei. I get an accurate count of nuclei and pretty good myotube mask but I am having a little trouble counting the total nuclei in myotubes. I saw a previous post about it using UnifyObjects to merge myotubes and I do not see that module now.
Any help would be much appreciated.
I currently am using the
3.) IdentifySecondaryObjects(Myotube,Nuclei as input,propagation,automatic, regularization 0.001)
4.) IdentifyTertiaryObjects(Myotube, Nuclei, named Cytoplasm)
I inlcuded some pictures and the pipeline I made
myotube_goodnuc.cpproj (98.4 KB)