Sample image and/or code
We have some images of GFP puncta captured with a spinning disk microscope.
One of the analysis that we’d like to perform on the image is to calculate the variance in intensity on a per pixel basis. We’re wondering what the best way to do this (either in ImageJ, or otherwise) would be.
- What stops you from proceeding? We’re just not sure where to start. If anyone can offer links or suggestions, or tips, that’d be really appreciated.