Sample image and/or code
The picture I have shared is that of nuclei (Blue regions) and Fluorescent spots within it (in Red). I am currently able to segment these Blue regions and red spots successfully and also calculate their mean fluorescence intensity. I have a relateObject with each of the fluorescent spots related to their respective blue regions.
I would like to additionally calculate also the ‘Integrated Intensity’ per blue region. As per my understanding, this means summing up the pixels per Blue region (which is my Region of Interest).
How might I calculate the Integrated Intensity of the combined Blue region and red spots per Blue region? Will measuring intensity directly on my relateObject (with both ROI and spots) work?