Hello to all,
Does anybody have a solution for my problem with Fiji?
- I opened RGB image and divide it in 3 channels, red, blue and green.
- Then i made one roi
- Then for example i take red channel and measure values of roi that i selected and fiji given me some values, for example mean value is 350
- Then i use multiply function for that R channel and multiply it by 2 and get new image.
- After that i used the same roi that i used in step 3 ( on regular image of R channel) and made measurements. The result for mean value was not 700, which i expected because 350x2 should be 700. It showed me different result it showed me 650.
So i hope that you understand me, and i would be happy if someone can help me.