You all are the experts, and I wish to ask you about image analysis to aid my pursuit for scientific truth. My issue seems simple but I think I am beginning to understand it may be complicated?
My goal: I would like to calculate stain intensities for all three channels (RGB) separately for a SINGLE selection. That is, I need to be able to make a single selection which is the exact same across all three channels, and analyze the respective intensities for it (i.e. red intensity, green intensity, blue intensity).
Is this possible for me to do and how would I go about producing these results?
Thank you very much in advance for your help on this matter.