I am trying to analyze lung tissue images with CellProfiler version 3.1.5. My goal is to quantify the distribution of GFP+ cells: I’d like to calculate the distance from airway to each GFP+ cell, or calculate how many GFP+ cells there are around each airway (Please see the images below). I could detect GFP+ cells (Identify Primary Objects) and each airway (Smooth > Enhance Edges > Enhance Features), but I’m not sure how to measure the distance from cells and airways.
Could you please give me some suggestions or advice?
Thank you for your help.