I am looking to analyze ratiometric calcium flux data and am wondering whether Cell Profiler is a suitable program to perform this type of analysis with? Basically I have about 20 views with 100 cells. I image the cells for about 60 time frames at 340 and 380nm. So ideally I’d like to apply a mask to the fluorescent images, have Cell Profiler integrate the fluorescence intensity over each cell and over all 60 time frames. I’d then like to take the ratio and plot the data. Is this possible with Cell Profiler and would you recommend using it for such an application?
It would be great if you could share your insights on this matter (and maybe flow processes if you have any). Thanks so much and many kind regards